Angiogenesis in vivo was further assayed using the dorsal air sac method (22) The associated drugs include albendazole, thiabendazole, riclabendazole, flubendazole, mebendazole and fenbendazole. Kralova, V.; Hanušová, V.; Caltová, K.; Špaček, P.; Hochmalová, M.; Skálová, L.; Rudolf, E. Flubendazole and mebendazole impair migration and epithelial to mesenchymal transition in oral cell lines. ; Williamson, T.; Riggins, G.J. to assay angiogenesis in vivo. 2G)⇓ Todorov, T.; Vutova, K.; Mechkov, G.; Georgiev, P.; Petkov, D.; Tonchev, Z.; Nedelkov, G. Chemotherapy of human cystic echinococcosis: Comparative efficacy of mebendazole and albendazole. Cancer Immunotherapy, Part 3: Challenges and Future Trends. These results suggest that MZ is effective in the treatment of cancer and other angiogenesis-dependent diseases. . Twenty-four h after each chamber was implanted, the animals were fed 1 mg of MZ p.o. Decrease in XIAP levels, increase in apoptosis markers (cleaved PARP and caspase 9) at 0.5 μM. Northcott, P.A. Targeting Cancer Stemness in the Clinic: From Hype to Hope. Further investigations are warranted to confirm the clinical anti-neoplastic activity of MBZ and its safety in combination with other drugs in a clinical setting. Cell invasion inhibition (0.085 μM). . For example, rapid, extensive metabolism of BZs into less toxic metabolites (e.g., sulfoxides and sulfones) by the hepatic microsomal enzymes (28 Mebendazole at 0.35 and 0.7 µM dose-dependent decrease of ALDH1 positive CSCs; Hedgehog pathway inhibition. Co, approximate tumor weight of ∼3–5 mm diameter tumor before starting MZ treatment; C, untreated and MZ-treated mice on day 28. Rubin, J.; Mansoori, S.; Blom, K.; Berglund, M.; Lenhammar, L.; Andersson, C.; Loskog, A.; Fryknäs, M.; Nygren, P.; Larsson, R. Mebendazole stimulates CD14+ myeloid cells to enhance T-cell activation and tumour cell killing. ; Pomeroy, S.L. demonstrated the antiproliferative effect of MBZ on human NSCLC cell lines (A549, H129, and H460) reporting an IC, The SHH signaling pathway, involving downstream effectors smoothened (SMO) and glioma-associated homolog 1 (GLI1), is constitutively activated in many types of cancer [, The effect of MBZ and flubendazole (FBZ) on migration and proliferation of PE/CA-PJ15 and H376 oral squamous carcinoma cells and premalignant oral keratinocytes DOK was tested by Kralova et al. Mebendazole Potentiates Radiation Therapy in Triple-Negative Breast Cancer. Moreover, MZ had no effect on normal endothelial cell growth but directly targeted tumor cells in vivo. Histochemical staining of lung tissues using H&E indicated that not only the number but also the size of the metastatic tumor colonies (as measured according to the transverse diameter of the tumor colony) was substantially reduced by treatment using MZ (Fig. ; Riggins, G.J. ; Van Eldik, L.J. The anti-cancer … . ; Dakshanamurthy, S. Repurpose VS: A Drug Repurposing-Focused Computational Method for Accurate Drug-Target Signature Predictions. Update December 2019: A recent study suggests that for Pancreatic Cancer, two other anti-worm drugs from the same category and used in animals, Parbendazol (brand name Verminum, Worm Guard and Helatac) and Oxibendazole, is more effective compared to Fenbendazole and Mebendazole (Ref.). Anticancer treatment efficacy is limited by the development of refractory tumor cells characterized by increased expression and activity of mechanisms promoting survival, proliferation, and metastatic spread. Autophagy Is a Potential Target for Enhancing the Anti-Angiogenic Effect of Mebendazole in Endothelial Cells. ↵1 Partially supported by grants from a developmental grant from the National Cancer Institute to The University of Texas M. D. Anderson Cancer Center; Specialized Program of Research Excellence Grant P50-CA70907 for lung cancer (to T. M.); the National Cancer Institute, NIH Grant P01 CA78778–01A1 (to J. mebendazole (MZ), a microtubule-disrupting anthelmintic that exhibits a potent antitumor property both in vitro and in vivo. Inhibition of VEGFR2 autophosphorylation, at 1–10 μM in cultured HUVECs and with an IC50 of 4.3 μM in a cell-free kinase assay. ; Confortin, G.; Junqueira, A.V. To exclude the possibility that the reduced vasculature was attributable to a lack of viable tumor cells in the chamber, we prelabeled tumor cells using a fluorescent dye before injecting them into the chamber. , 24) Next, DNA fragments were precipitated with 0.5 m NaCl and 50% isopropanol, and the samples were loaded in 2% agarose TBE gel and stained with ethidium bromide.